dapi ii antifade solution (Abbott Laboratories)
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Abbott Laboratories
dapi ii antifade solution
Dapi Ii Antifade Solution, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution/antifade+solution/pmc13280351-69-4-8
Average 86 stars, based on 1 article reviews
Dapi Ii Antifade Solution, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution/antifade+solution/pmc13280351-69-4-8
Average 86 stars, based on 1 article reviews
dapi ii antifade solution - by Bioz Stars,
2026-09
86/100 stars
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Incubation:Article Title: F 4 -Neuroprostane Effects on Human Sperm. Article Snippet: .. After this, samples were incubated with 1 μg/mL PSA (in PBS) for 30 min, washed with PBS for 15 min, and nuclei were stained with Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Tissues of brain, heart, lung, spleen, liver and kidney with positive nested-PCR results were subjected to frozen section at the slice thickness of 4 μm; the slices were air-dried and then OCT gel was removed; to improve adhesion of tissue slices and to adequately spread tissue structures, cytospin (Shandon, Thermo Electron Corporation, UK) was performed at 2,000 rpm and moderate acceleration over 2 min; (the following processes are to be performed light-shielded) slices were incubated with FITC-antibody (directly labeled) or primary antibody (indirectly labeled) at room temperature for 40 min (performed according to the procedures provided by reagent manufacturers); rinsed 3-4 times with PBS pre-cooled at 6-8°C; for indirectly labeled antibody, incubation was continued on with FITC-second antibody at room temperature for 30 min (performed according to the procedures provided by reagent manufacturers); rinsed 3-4 times with PBS pre-cooled at 6-8°C. .. After incubation with antibodies, tissue slices were to be air-dried; soaked in 2×SSC/0.1%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) preheated at 37 for 30 min; excessive solution on the slices was removed; and the slices were subjected to gradient dehydration in 70%, 85% and 100% ethanol at room temperature, with 2 min in each gradient; slices were air-dried, added with 10 μL human-Y specific probe mixture (prepared based on the regimen provided by Abbott Molecular Inc. USA); covered with a cover slip, the slices underwent co-denaturation at 73±1 in 6-7 min; and then placed in a sealed humid container pre-heated at 42 for 16 hours; rinsed in 2×SSC/0.3%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) preheated at 73±1 over 2 min; transferred into 2×SSC/0.3%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) at room temperature for 2 min; excessive solution on the slices was removed and the slices air-dried, counterstained by dripping 10-15 μL Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Cover slips adhered by cultured cells were gently rinsed thrice with PBS preheated at 36.8±0.2 ; cover slips used in immunohistochemical DAB staining of Nestin, Vimentin, β- tubulin, GFAP, CK19, CD10, CD3, CD19, CD16, CD45, CD34, CD31 and VEGF R2 (abcam, USA & Invitrogen, USA) were fixed using methanol over 20 min and then slightly rinsed with distilled water; subjected to 3%Triton x-100 over 20 min at room temperature and then slightly rinsed with PBS; treated with 3%H2O2 over 10 min at room temperature and again slightly rinsed with PBS; blocked with normal goat serum over 10 min, and were dried by shaking them; then corresponding primary antibody was added, followed by incubation at 37 for one hour and rinsing with PBS thrice, with 3 min in each time; A solution (intensifier) was added drop by drop, followed by incubation at 37 for 15 min; Again the cover slips were rinsed with PBS thrice, with 3 min in each time; B solution (Polymerized HRP-Anti Ms/Rb IgG) was added drop by drop, followed by incubation at 37 for 20 min and rinsing with PBS thrice, with 3 min in each time; DAB was added to develop the color over 10 min; the cover slips were then observed under microscope, and rinsed with tap water to terminate the reaction; counterstaining was achieved using Mayer`s hematoxylin over 1 min, followed by rinsing in tap water to blue the stain; the cover slips were left to dry, sealed and observed and photographed under regular optical microscope (Zeiss, Germany). .. Cover slips adhered by cultured cells used in immunohistochemical staining of CD90, CD73, CD105 (Santa Cruz, USA), HLA-DR and HLA-I (Invitrogen,USA) were rinsed with PBS, and immediately incubated with FITC-labeled antibody according to the protocol provided by reagent manufacturers, and then fixed over 1 min using pure methanol and glacial acetic acid at the ratio of 3: 1 pre-cooled at 4 ; They were then left to dry and counterstained with Article Title: In Vivo Transplantation of Neurosphere-Like Bodies Derived from the Human Postnatal and Adult Enteric Nervous System: A Pilot Study Article Snippet: .. After washing, slides were air-dried for 20 min in the dark, incubated with Staining:Article Title: F 4 -Neuroprostane Effects on Human Sperm. Article Snippet: .. After this, samples were incubated with 1 μg/mL PSA (in PBS) for 30 min, washed with PBS for 15 min, and nuclei were stained with Article Title: F 4 -Neuroprostane Effects on Human Sperm. Article Snippet: The next day, the samples were washed 3 times in PBS with 0.1% Tween 20 and treated with a secondary anti-rabbit antibody raised in goat Alexa Fluor® 488 conjugate (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) diluted 1:500 for 1 h at room temperature. .. Nuclei were stained with Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Cover slips adhered by cultured cells were gently rinsed thrice with PBS preheated at 36.8±0.2 ; cover slips used in immunohistochemical DAB staining of Nestin, Vimentin, β- tubulin, GFAP, CK19, CD10, CD3, CD19, CD16, CD45, CD34, CD31 and VEGF R2 (abcam, USA & Invitrogen, USA) were fixed using methanol over 20 min and then slightly rinsed with distilled water; subjected to 3%Triton x-100 over 20 min at room temperature and then slightly rinsed with PBS; treated with 3%H2O2 over 10 min at room temperature and again slightly rinsed with PBS; blocked with normal goat serum over 10 min, and were dried by shaking them; then corresponding primary antibody was added, followed by incubation at 37 for one hour and rinsing with PBS thrice, with 3 min in each time; A solution (intensifier) was added drop by drop, followed by incubation at 37 for 15 min; Again the cover slips were rinsed with PBS thrice, with 3 min in each time; B solution (Polymerized HRP-Anti Ms/Rb IgG) was added drop by drop, followed by incubation at 37 for 20 min and rinsing with PBS thrice, with 3 min in each time; DAB was added to develop the color over 10 min; the cover slips were then observed under microscope, and rinsed with tap water to terminate the reaction; counterstaining was achieved using Mayer`s hematoxylin over 1 min, followed by rinsing in tap water to blue the stain; the cover slips were left to dry, sealed and observed and photographed under regular optical microscope (Zeiss, Germany). .. Cover slips adhered by cultured cells used in immunohistochemical staining of CD90, CD73, CD105 (Santa Cruz, USA), HLA-DR and HLA-I (Invitrogen,USA) were rinsed with PBS, and immediately incubated with FITC-labeled antibody according to the protocol provided by reagent manufacturers, and then fixed over 1 min using pure methanol and glacial acetic acid at the ratio of 3: 1 pre-cooled at 4 ; They were then left to dry and counterstained with Article Title: Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line Article Snippet: Subsequently, the cell specimens were washed with 0.4X SSC buffer containing 0.3% Nonidet (N)P-40 (Abbott Molecular) at 73°C for 2 min, followed by 1-min wash at RT with 2X SSC buffer containing 0.1% NP-40. .. Next, the specimens were dried in the dark at RT, stained with 10 μl 125 ng/ml Microscopy:Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Tissues of brain, heart, lung, spleen, liver and kidney with positive nested-PCR results were subjected to frozen section at the slice thickness of 4 μm; the slices were air-dried and then OCT gel was removed; to improve adhesion of tissue slices and to adequately spread tissue structures, cytospin (Shandon, Thermo Electron Corporation, UK) was performed at 2,000 rpm and moderate acceleration over 2 min; (the following processes are to be performed light-shielded) slices were incubated with FITC-antibody (directly labeled) or primary antibody (indirectly labeled) at room temperature for 40 min (performed according to the procedures provided by reagent manufacturers); rinsed 3-4 times with PBS pre-cooled at 6-8°C; for indirectly labeled antibody, incubation was continued on with FITC-second antibody at room temperature for 30 min (performed according to the procedures provided by reagent manufacturers); rinsed 3-4 times with PBS pre-cooled at 6-8°C. .. After incubation with antibodies, tissue slices were to be air-dried; soaked in 2×SSC/0.1%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) preheated at 37 for 30 min; excessive solution on the slices was removed; and the slices were subjected to gradient dehydration in 70%, 85% and 100% ethanol at room temperature, with 2 min in each gradient; slices were air-dried, added with 10 μL human-Y specific probe mixture (prepared based on the regimen provided by Abbott Molecular Inc. USA); covered with a cover slip, the slices underwent co-denaturation at 73±1 in 6-7 min; and then placed in a sealed humid container pre-heated at 42 for 16 hours; rinsed in 2×SSC/0.3%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) preheated at 73±1 over 2 min; transferred into 2×SSC/0.3%NP-40 solution (prepared based on the regimen provided by Abbott Molecular Inc. USA) at room temperature for 2 min; excessive solution on the slices was removed and the slices air-dried, counterstained by dripping 10-15 μL Fluorescence:Article Title: F 4 -Neuroprostane Effects on Human Sperm. Article Snippet: The next day, the samples were washed 3 times in PBS with 0.1% Tween 20 and treated with a secondary anti-rabbit antibody raised in goat Alexa Fluor® 488 conjugate (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) diluted 1:500 for 1 h at room temperature. .. Nuclei were stained with Cell Culture:Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Cover slips adhered by cultured cells were gently rinsed thrice with PBS preheated at 36.8±0.2 ; cover slips used in immunohistochemical DAB staining of Nestin, Vimentin, β- tubulin, GFAP, CK19, CD10, CD3, CD19, CD16, CD45, CD34, CD31 and VEGF R2 (abcam, USA & Invitrogen, USA) were fixed using methanol over 20 min and then slightly rinsed with distilled water; subjected to 3%Triton x-100 over 20 min at room temperature and then slightly rinsed with PBS; treated with 3%H2O2 over 10 min at room temperature and again slightly rinsed with PBS; blocked with normal goat serum over 10 min, and were dried by shaking them; then corresponding primary antibody was added, followed by incubation at 37 for one hour and rinsing with PBS thrice, with 3 min in each time; A solution (intensifier) was added drop by drop, followed by incubation at 37 for 15 min; Again the cover slips were rinsed with PBS thrice, with 3 min in each time; B solution (Polymerized HRP-Anti Ms/Rb IgG) was added drop by drop, followed by incubation at 37 for 20 min and rinsing with PBS thrice, with 3 min in each time; DAB was added to develop the color over 10 min; the cover slips were then observed under microscope, and rinsed with tap water to terminate the reaction; counterstaining was achieved using Mayer`s hematoxylin over 1 min, followed by rinsing in tap water to blue the stain; the cover slips were left to dry, sealed and observed and photographed under regular optical microscope (Zeiss, Germany). .. Cover slips adhered by cultured cells used in immunohistochemical staining of CD90, CD73, CD105 (Santa Cruz, USA), HLA-DR and HLA-I (Invitrogen,USA) were rinsed with PBS, and immediately incubated with FITC-labeled antibody according to the protocol provided by reagent manufacturers, and then fixed over 1 min using pure methanol and glacial acetic acid at the ratio of 3: 1 pre-cooled at 4 ; They were then left to dry and counterstained with Immunohistochemical staining:Article Title: Generation of Human Epidermis-Derived Mesenchymal Stem Cell-like Pluripotent Cells and their reprogramming in mouse chimeras Article Snippet: Cover slips adhered by cultured cells were gently rinsed thrice with PBS preheated at 36.8±0.2 ; cover slips used in immunohistochemical DAB staining of Nestin, Vimentin, β- tubulin, GFAP, CK19, CD10, CD3, CD19, CD16, CD45, CD34, CD31 and VEGF R2 (abcam, USA & Invitrogen, USA) were fixed using methanol over 20 min and then slightly rinsed with distilled water; subjected to 3%Triton x-100 over 20 min at room temperature and then slightly rinsed with PBS; treated with 3%H2O2 over 10 min at room temperature and again slightly rinsed with PBS; blocked with normal goat serum over 10 min, and were dried by shaking them; then corresponding primary antibody was added, followed by incubation at 37 for one hour and rinsing with PBS thrice, with 3 min in each time; A solution (intensifier) was added drop by drop, followed by incubation at 37 for 15 min; Again the cover slips were rinsed with PBS thrice, with 3 min in each time; B solution (Polymerized HRP-Anti Ms/Rb IgG) was added drop by drop, followed by incubation at 37 for 20 min and rinsing with PBS thrice, with 3 min in each time; DAB was added to develop the color over 10 min; the cover slips were then observed under microscope, and rinsed with tap water to terminate the reaction; counterstaining was achieved using Mayer`s hematoxylin over 1 min, followed by rinsing in tap water to blue the stain; the cover slips were left to dry, sealed and observed and photographed under regular optical microscope (Zeiss, Germany). .. Cover slips adhered by cultured cells used in immunohistochemical staining of CD90, CD73, CD105 (Santa Cruz, USA), HLA-DR and HLA-I (Invitrogen,USA) were rinsed with PBS, and immediately incubated with FITC-labeled antibody according to the protocol provided by reagent manufacturers, and then fixed over 1 min using pure methanol and glacial acetic acid at the ratio of 3: 1 pre-cooled at 4 ; They were then left to dry and counterstained with Fluorescence In Situ Hybridization:Article Title: Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix Article Snippet: The UroVysion Bladder Cancer Kit (Abbott Molecular) which contains DNA Probe Mixture (Fluorophore-labelled DNA probes for chromosomes 3, 7, and 17, and locus 9p21 in hybridization buffer), DAPI II Counterstain, 20X SSC, NP-40 (non-ionic detergent). b. .. The Vysis FISH Pretreatment Reagent Kit (Abbott Molecular) which includes Vysis Protease solution (3 × 25 mg) with Pepsin Activity, Vysis Pepsin Buffer (3 × 50 mL) 10 mM HCl, Vysis PBS 1X (2 × 250 mL), Vysis 20X SSC (66 g), 10% neutral buffered formalin and Activity Assay:Article Title: Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix Article Snippet: The UroVysion Bladder Cancer Kit (Abbott Molecular) which contains DNA Probe Mixture (Fluorophore-labelled DNA probes for chromosomes 3, 7, and 17, and locus 9p21 in hybridization buffer), DAPI II Counterstain, 20X SSC, NP-40 (non-ionic detergent). b. .. The Vysis FISH Pretreatment Reagent Kit (Abbott Molecular) which includes Vysis Protease solution (3 × 25 mg) with Pepsin Activity, Vysis Pepsin Buffer (3 × 50 mL) 10 mM HCl, Vysis PBS 1X (2 × 250 mL), Vysis 20X SSC (66 g), 10% neutral buffered formalin and |